![]() In addition, we added a faster imageio-based image loader to supplement the existing bioformats-based implementation ( Silvester et al., 2020). We also revised the program’s builds to package all Java dependencies within the main installer, which dramatically simplifies the installation process. We ported CellProfiler Analyst to the Python 3 programming language to ensure compatibility with future operating systems after the official Python 2 end-of-life in 2020. Herein we present CellProfiler Analyst 3.0, which includes major performance improvements and new features that improve the utility of the software. These tools provide an intuitive interface for scientists to explore their data in forms such as histograms and scatter plots, though without the advanced statistical tools or extreme customizability of a pure programming language. While other tools such as Ilastik ( Berg et al., 2019) and Advanced Cell Classifier ( Piccinini et al., 2017) can provide a GUI for training classifiers with image-based data, these do not include data exploration and visualization tools like those in CellProfiler Analyst. The software includes several tools for users to visualize and filter their datasets, alongside tools for training machine learning classifier models within a convenient graphical user interface that is geared toward working with image data ( Dao et al., 2016). CellProfiler Analyst is a data exploration package for helping users to explore and extract information from large datasets, including (but not limited to) those produced by CellProfiler pipelines ( Jones et al., 2008). Spreadsheet programs are familiar but lack features and integration with source images that biologists often need. Free software packages such as ImageJ ( Schneider et al., 2012) and CellProfiler ( McQuin et al., 2018) allow users to extract hundreds or thousands of numerical measurements from their image data, but it is ultimately on the user to determine which of these features are relevant to the biological problem being investigated. This necessitates automated computational analysis to efficiently derive biological insights from the raw data. ![]() With the increasing adoption of high-throughput microscopy, scientists have been able to generate large datasets containing thousands of individual images.
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![]() 1862 FALL – Frank James joins Quantrill’s Confederate Partisan Rangers (guerrillas). Reuben Samuel and Zerelda Cole James Simms Samuel. 1861 25 DEC – John Thomas Samuel, Frank and Jesse’s half-brother, born to Dr. Hite (Wood Hite’s brother) born in Logan County, Kentucky to George B. 1861 4 MAY – Frank James joins the Confederate Army. ![]() James lives on the family farm in Clay County, Missouri. Andruss Courtney in Johnson County, Warrensburg, Missouri. Courtney, age 14, lives in the household of his parents Stephen and Dianah D. 1858 26 DEC – Sarah Louisa Samuel, Frank and Jesse James’ half-sister, born to Dr. 1855 25 SEP- Zerelda Cole James Simms marries Dr. 1854 2 JAN – Benjamin Simms killed in a horse accident. 1852 30 SEP – Zerelda Cole James marries Benjamin Simms. Courtney, four-years-old, is not listed on census records for the household of his parents, Stephen and Dianah Courtney, in Johnson County, Missouri. 1850 25 NOV- Susan Lavinia James born to Robert Sallee James and Zerelda Cole. 1850 – Wood Hite, Frank and Jesse James’ paternal first cousin, born in Logan County, Missouri to George B. 1850- Robert Sallee James goes to the gold fields of California with a group of his parishioners. Robert Sallee James and Zerelda Cole James, in Kearney, Clay County, Missouri. 1850- Jesse James is living with his parents, Rev. Robert Sallee James reportedly dies in a mining camp called Rough and Ready, (since renamed Placerville) California, and is hastily buried in an unmarked grave. 1847 5 SEP- Jesse Woodson James born to Robert Sallee James, an ordained Baptist preacher, and Zerelda Cole on the family farm in Kearney, Clay County, Missouri. Courtney born in Washington, Tennessee to Stephen Courtney and Dianah D. ![]() Robert Sallee James and Zerelda Cole in Clay County, Missouri. Third, it is accepted as being self-evident.” The truth about Jesse James was first published in Texas Monthly magazine’s August 1997 issue and since that time it has been ridiculed and violently opposed, but, thanks to the recently discovered eBay photograph of the James family and the 1921 Quantrill Reunion photograph, it has now become self-evident.ġ843- Alexander Franklin “Frank” James born to Rev. This author has relied heavily on primary source “first hand” evidence including documents, personal diaries and photographs, instead of secondary literature offering second or third hand information.Īrthur Schopenhauer’s quote has proved to be true in the case of Jesse James: “All truth passes through three stages. Due to the eBay photograph of the James family providing visual proof that Jesse Woodson James got away with his own murder, the following chronicle is the most accurate one currently available. ![]() Due to their large size and longer-than-average lifespan, boa constrictors are recommended for intermediate and advanced snake keepers. ![]() Boa Constrictor Care Levelīoth the Common boa and the Colombian boa make great pets for herpetoculturists. Argentine Boa ConstrictorsĪrgentine's have between 242 and 251 ventral scales, 29-30 interconnected dorsal blotches, and black and white peppering. There are very few of this type of boa in existence. Both the posterior body and tail blotches are black, thus the name. It has dark, interconnected saddling on a background color that ranges from yellow-brown to light powder-gray, with copious amounts of black throughout. This is a distinct morph (morph is variety created by breeders and not found in the wild) with a wide, central head stripe and black anterior and posterior facial markings. They have flatter-looking heads and a background color that ranges from gray and silver to a rich, dusky yellow. Short-tailed Boas have between 226 and 237 ventral scales, twenty-two dorsal blotches, and heavily speckled dark bellies. Red-Tailed Boas are the largest subspecies of boa and are arguably the most beautiful. They have black speckled bellies and defined red blotches on the surface of their posterior and tail, thus the name. This is the most popular boa subspecies, with a greater number of ventral scales (234–250) and blotches. There are quite a few different color and pattern varieties created by breeders, one of the most popular being the scarlet Blood Phase Boa. Common boas don’t have speckled bellies, but if they do they are lightly speckled and the pattern on the tail appears to be smudged. ![]() The common boas have less than 253 ventral (belly surface) scales and at least 21 dorsal (top surface) blotches (the area between the vent and the neck). They typically have blotches on their tails and scale patterns that look like leaves, ovals, diamonds, or circles.ĭepending on the subspecies, your boa can take on a variety of looks. Depending on the habitat they’re trying to blend into, boas can have a range of colors, including tan, green, red, and yellow. A boa’s small cloacal spurs on the sides of its vent (anus) are actually connected to said vestigial limbs!īoa constrictors have distinctive and varied markings. They have vestigial remnants of the pelvis and hind limbs of their lizard-like predecessors. Boa Constrictor Appearanceīoas are primitive snakes, just like pythons. In fact, the oldest recorded boa lived to be just over forty-three and lived at the Philadelphia Zoological Gardens. It’s quite normal for a boa constrictor to live for twenty to thirty years or more. Boa Constrictor Lifespanīoa constrictors are among the longest-lived snake species in the world. The largest boa constrictor on record is believed to have been 14 feet in length. Most captive-bred boa constrictors do not exceed a weight of 60 pounds, with most subspecies topping out at around 30 pounds. Male boa constrictors tend to be a little smaller than females. The average adult size ranges from 4 to 7 feet in length, with some individuals occasionally reaching 8 or 9 feet in length. Newborn boa constrictors range in length between 14 and 22 inches and weigh just 2 to 3 ounces. Boa Constrictor Sizeīoas are one of the few snakes that produce live births, versus eggs that need to be incubated. There are six subspecies currently recognized by all herpetologists, including the Common Boa Constrictor, Red-Tailed Boa Constrictor, Clouded Boa Constrictor, San Lucia Boa Constrictor, Short-Tailed Boa Constrictor, and Argentine Boa Constrictor. This is one of the few instances where an animal’s scientific name is the same as its common name. For analysis of small or very simple experiments, spreadsheet programs like Microsoft Excel are sufficient, and useful open-source tools exist for analysis and exploration of data from high throughput screens in general. The volume and richness of individual-cell data from large image-based screens is unprecedented and existing software is inadequate for the challenge of data analysis. This tool has been useful for extracting image-based measurements to score sophisticated screens, with many more in progress. ![]() We recently developed open-source image analysis software, CellProfiler, which measures a rich set of cellular features in images, such as size, shape, and staining patterns including intensity, texture, and colocalization. ![]() This capability is increasingly important given the ease now to systematically perturb cells with libraries of chemicals or gene-perturbing reagents like RNA interference or gene overexpression and collect hundreds of thousands of images of these cell samples. The scientific community has only begun to scratch the surface of computationally extracting the rich information visible in fluorescence microscopy images of cell samples. Should you know of anything that we have not listed here let us know.Visual analysis of cell samples has played a dominant role in the history of biology. Download Free Expander AU VST Plugins & VSTi Instruments Here is our colection of FREE software, VST plugins, VSTi instruments, audio utilities and DAWs. TextExpander for Mac v6.5+ macOS 10.12, 10.11, 10.10 (Sierra, El Capitan, Yosemite) TextExpander for Mac v6.2.8: Windows 8, 10: TextExpander for Windows v2: Chrome v73 or later: TextExpander for Chrome v1: iOS 9 or later: TextExpander for iPhone and iPad v4. StuffIt Expander opens files created with WinZip®, 7zX, iShrink, SimplyRAR, Rarify, Rucksack (formerly iArchiver), BetterZip, RarMachine, GUI Tar, CleanArchiver, Springy, TarPit, RAR, Ez7z, Keka, and the compression utilities built into recent. From BinHex to Zip, StuffIt to MIME, Expander accesses more formats, in less time, with zero hassles. ![]() ![]() Free Manga Downloader The Free Manga Downloader (FMD) is an open source application written in Object-Pascal for managing. I'm not sure why - it's not as if they're losing out in some way - you'd think it would be a case of the more the merrier, wouldn't you? And no, we're not leet haxx0rz, NamcoBandai have, for the first time, released this head-punching beast on both the PS3 and 360.Īnd the PS3 fan boys aren't happy. Yes, we're playing Tekken 6 and yes, we're playing it on a 360. ![]() The stages go through dynamic changes ranging from a sudden attack helicopter crash to vehicle explosions! Original Characters Returning Are: Anna Williams Armor King Asuka Kazama Baek Doo San Bruce Irvin Bryan Fury Christie Monteiro Craig Marduk Jack-6 Devil Jin Eddy Gordo Feng Wei Ganryu Heihachi Mishima Hwoarang Jin Kazama Julia Chang Kazuya Mishima King Kuma LeeChaolan Lei Wulong Lili Ling Xiaoyu Marshall Law Mokujin Nina Williams Panda Paul Phoenix Raven Roger Jr.Eagle-eyed viewers will notice an immediate discrepancy with the things they've just seen. Then take your own ultimate fighters online! Dynamic, Interactive, Destructible Environments: Punch your enemies through concrete walls or kick them through floors to open a whole new battleground. Customise their hairstyles, apparel, fashion accessories, tattoos, etc. The Largest Character Roster Ever: Master 40 unique fighters representing the world's top martial arts styles! The character line-up includes 34 beloved characters, such as Jin, Heihachi and Kazuya, as well as 6 brand new characters with unique fighting styles and combo systems! Compete and Battle Globally: Prove your supremacy against players from around the world with Online VS Mode! Endless Possibilities to Customise: Create new characters and customise them with every imaginable detail. The stages go through dynamic changes ranging from a sudden attack helicopter crash to vehicle explosions! Features: Gorgeous Graphics: The advanced high-definition graphics and 3D animation technologies create the most impressive HD visuals in Tekken history. ![]() Then take your own ultimate fighters online!Dynamic, Interactive, Destructible Environments: Punch your enemies through concrete walls or kick them through floors to open a whole new battleground. With new dynamic, interactive and destructible Environments, punching your enemies through concrete walls or through floors can open a whole new battleground.Gorgeous Graphics: The advanced high-definition graphics and 3D animation technologies create the most impressive HD visuals in Tekken history.The Largest Character Roster Ever: Master 40 unique fighters representing the world's top martial arts styles! The character line-up includes 34 beloved characters, such as Jin, Heihachi and Kazuya, as well as 6 brand new characters with unique fighting styles and combo systems!Compete and Battle Globally: Prove your supremacy against players from around the world with Online VS Mode!Endless Possibilities to Customise: Create new characters and customise them with every imaginable detail. Prove your supremacy against players from around the world with online VS mode, create new characters and customize them with every imaginable detail. ![]() Armed with new HD graphics running flawlessly at 60 fps the largest character roster ever in Tekken gaming history ground-breaking online game modes and robust character customization system the greatest fighting game franchise soars onto the next generation of consoles.Master 40 unique fighters representing the worlds top martial arts styles, including 34 beloved characters, such as Jin, Heihachi and Kazuya, as well as 6 brand new characters with unique fighting styles and combo systems. ![]() You can choose between red-cyan, green-magenta or yellow-blue lenses. Stereoscopic imaging option (requires anaglyph eyeglasses).Camera tilt only works in landscape mode. Camera tilt brings dimensionality to the games offering a simulated 3D effect where the image changes depending upon how you tilt the device.Unique soundtrack with atmospheric music, sound effects and voices.At the end of each game, you are prompted to enter your name and have your score included. Swiping right brings up a table of local high scores and further tapping brings up high scores from copies sold throughout Make sure to swipe left and right when viewing since the instructions don't fit on one screen. A wonderful 3D engine that does true justice to the graphic capabilities of the high-end mobile devices.Portrait mode gives you a flying-table view with the camera panning and zooming over the action.The games can be played in either landscape orientation with the button to the left or right of the screen. Landscape mode shows a full table view. ![]() 12 pinball tables with unique graphics, instructions, missions system, locations and characters.In portrait mode slide up or down your both thumbs to toggle camera view from "flying-table" to "full table".This is a REAL Pinball simulator with stunning 3D graphics both with or without 3D glasses. You'll be stunned by the level of realism. Each table - Wild West, The Deep, Jungle Style, Snow, Da Vinci, Arcade, Fantasy, Zombie Attack, Pirates, Tanks, Street Racing - are masterpieces. Pinball HD Collection - This game will change your idea of Pinball on the Android. This unique table features pinball bumpers and targets, plus a color monitor where you can take on a variety of old-school digital minigame challenges - an endless space scroller, plus car racing, missile defense, a block breaker, and more at incrementing skill levels. Google Compute Engine instance) and specify the '-L' flag. Second, establish an SSH connection from your local machine to the remote instance (e.g. If you use these images, skip directly to Step 4: Connect to the local runtime (using port 8888).įirst, set up your Jupyter notebook server using the instructions above. Follow the how-to guides to set up your Google Compute Engine instance with local SSH port forwarding. ![]() Note: Google Cloud Platform provides Deep Learning VM images with Colaboratory local backend support preconfigured. Google Compute Engine instance), you can set up SSH local port forwarding to allow Colaboratory to connect to it. If the Jupyter notebook server you'd like to connect to is running on another machine (e.g. Jupyter serverextension disable -py jupyter_http_over_wsĬonnecting to a runtime on a Google Compute Engine instance You can disable and remove the jupyter_http_over_ws jupyter extension by running the following: If your local connection will access sensitive data and you would like to omit code cell outputs, select Edit > Notebook settings > Omit code cell output when saving this notebook. When others open the shared notebook, they will be connected to a standard Cloud runtime by default.īy default, all code cell outputs are stored in Google Drive. If you share your notebook with others, the runtime on your local machine will not be shared. By default, Firefox disallows connections from HTTPS domains using standard WebSockets. Colaboratory makes a connection to your local kernel using a WebSocket. Note: If you're using Mozilla Firefox, you'll need to set the preference within the Firefox config editor. After this, you should now be connected to your local runtime. Enter the URL from the previous step in the dialog that appears and click the "Connect" button. In Colaboratory, click the "Connect" button and select "Connect to local runtime.". Make a copy of this URL as you'll need to provide this in the next step. Once the server has started, it will print a message with the initial backend URL used for authentication. New notebook servers are started normally, though you will need to set a flag to explicitly trust WebSocket connections from the Colaboratory frontend. Jupyter serverextension enable -py jupyter_http_over_ws ![]() The jupyter_http_over_ws extension is authored by the Colaboratory team and available on GitHub. ![]() Step 2: Install and enable the jupyter_http_over_ws jupyter extension (one-time) In order to allow Colaboratory to connect to your locally running Jupyter server, you'll need to perform the following steps. For more information on the Jupyter notebook server's security model, consult Jupyter's documentation. " rm -rf /")īefore attempting to connect to a local runtime, make sure you trust the authors of the notebook and ensure you understand what code is being executed. By connecting to a local runtime, you are allowing the Colaboratory frontend to execute code in the notebook using the local resources on your machine. With these benefits come serious potential risks. With a local connection, the code you execute can read, write, and delete files on your computer.Ĭonnecting to a Jupyter notebook server running on your local machine can provide many benefits. Make sure you trust the authors of any notebook before executing it. This allows you to execute code on your local hardware and have access to your local file system. Colaboratory lets you connect to a local runtime using Jupyter. ![]() ![]() In fact, it's implied that they were puppets all along, adding to the whole creepy carnival aesthetic of the troupe. The first thing you'll notice are the masks of the audience who were watching your fights against Troupe Master Grimm, suggesting that all was not as it seemed. Finding this area makes you feel like you've stumbled upon an eerie place unintended for prying eyes. If you decide to do the Grimm Troupe DLC, there's a room that is devilishly hidden inside the sinister tents that pop up on the outskirts of Dirtmouth (much to Elderbug's displeasure). Though there are many other difficult-to-find passageways that grant access to treasures, upgrades, and hot springs (such as the one in the Colosseum of Fools), these obscure lore rooms remain the holy grail of Hallownest finds. Hidden from plain sight, most of these secret rooms grant an unrivaled glimpse into Hollow Knight's cryptic lore and are well-worth seeing for this quality alone. RELATED: Hollow Knight: Pieces Of Cut Content Even the most thorough of completionists are likely to remain blissfully unaware of some of the secrets Hollow Knight has to offer. Like the illusory walls of Dark Souls, some of these aren't clearly signposted, if at all, and it can be easy to miss more than a few of them along your travels. Seeing everything that the game has to offer is made into a stupendous task, however, by the existence of breakable walls and well-hidden passageways. The world of Hollownest is ripe for exploration, with new content waiting around almost every corner. ![]() So to get started making this easy chocolate fudge, you’ll need just three ingredients – chocolate chips, sweetened condensed milk and vanilla extract. Not to mention, it’s a great chocolate fix if you’re losing your mind a bit and chocolate makes you feel better. And if you’re not fully prepared and need something quick, this fudge is for you. When did that happen?!? I’m so not ready. So much going on, so little time and oh my gosh, Christmas is like 10 days away. □ The only problem with that is it might be too easy.īut of course easy is exactly what we need this time of year. And two – it’s a delicious treat to snack on when taking care of twins. Now, there are two great things I’ve found about fudge lately. Did you catch my Easy White Chocolate Cranberry Fudge recently? SO good! Both that fudge and this one are so rich and creamy, it’s hard to stop eating them! I’ve been loving testing out new fudge recipes lately.
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